Journal: Frontiers in Immunology

Article Title: TGF-β and TNF-α interaction promotes the expression of MMP-9 through H3K36 dimethylation: implications in breast cancer metastasis

doi: 10.3389/fimmu.2024.1430187

Figure Lengend Snippet: MMP-9 expression is associated with TGF-β and TNF-α in breast cancer tissues. Immunohistochemical staining was done for MMP-9, TGF-β, and TNF-α expressions. Tissue microarray TMA slides (88 breast cancer tissues from breast cancer patients) were subjected to immunohistochemistry using antibodies against MMP-9, TGF-β, and TNF-α. Based on IHC staining intensities of TGF-β and TNF-α, patients’ samples were divided into four groups (Low TNF-α- Low TGF-β; Low TNF-α- High TGF-β; High TNF-α- Low TGF-β; High TNF-α- High TGF-β. (A) Scatter Plot Graph for TNF-α and TGF-β. (B) Scatter Plot Graph for TNF-α and TGF-β with indication of patients with primary cancer, metastasis and normal breast cancer tissues. (C) MMP-9 expression was elevated in the breast cancer tissues (n=24 patients) having high levels of TGF-β and TNF-α compared to the tissues (n=20 patients) having less TGF-β and TNF-α. (D) Representative IHC images of the samples of breast cancer tissues with low levels of MMP-9, TGF-β and TNF-α. (E) Representative IHC images of the samples of breast cancer tissues showing high levels of MMP-9, TGF-β and TNF-α. All data is presented as the mean ± SD. *p < 0.05.

Article Snippet: We purchased commercial breast cancer tissue microarray (TMA) from US Biomax Inc.(BRM961a) https://www.tissuearray.com/tissue-arrays/Breast/BRM961b ; TissueArray.Com LLC 1(5885 Crabbs Branch Way, Derwood, MD 20855, USA.

Techniques: Expressing, Immunohistochemical staining, Staining, Microarray, Immunohistochemistry

Journal: bioRxiv

Article Title: Loss function of tumor suppressor FRMD8 confers resistance to tamoxifen therapy via a dual mechanism

doi: 10.1101/2024.08.01.606147

Figure Lengend Snippet: (A) Lysates from human mammary epithelial cell (MEC) and breast cancer cells were subjected to immunoblotting. (B) Dot plot showing the expression of Frmd8 in epithelial cell linages from PyMT mice. (C) Methylation of FRMD8 promoter region in breast cancer according to the University of California Santa Cruz (UCSC) database ( http://xena.ucsc.edu/ ). (D) SUM159 and MDA-MB-231 cells were treated with 5-Aza-dC (10 μM) for 48 h. Protein expression of FRMD8 was examined by Western blot. (E) SUM159 and MDA-MB-231 cells were treated with 5-Aza-dC (10 μM) for 48 h. FRMD8 mRNA levels was examined by quantitative reverse transcription PCR (qRT-PCR) GAPDH was used as an internal reference. ** p <0.01 by unpaired Student’s t-test. (F) IHC analysis of FRMD8 expression in human breast carcinoma TMA was performed. Representative examples (scale bar, 100 μm) of normal tissue adjacent to tumor and breast cancer with different levels of FRMD8 expression are shown, with the magnification of selected areas inserted. (G) Kaplan-Meier analysis for the overall survival of breast cancer patients according to FRMD8 expression (Log-rank test). (H-J) Recurrence free survival of breast cancer patients according to FRMD8 expression were analyzed according to Kaplan-Meier plotter ( http://kmplot.com/analysis/ ).

Article Snippet: Human breast carcinoma TMA (HBre-Duc140Sur-01) was purchased from Shanghai Biochip.

Techniques: Western Blot, Expressing, Methylation, Reverse Transcription, Quantitative RT-PCR